CRYGA sirna

CRYGA siRNA (Mouse)

Cat. Num. AAA828423

• Gene Names: Cryga; Cryg-4; DGcry-4
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRYGA; CRYGA siRNA (Mouse); Gamma-crystallin A; Gamma-A-crystallin; Gamma-crystallin 4; CRYGA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CRYGA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 174

• Applicable Applications for CRYGA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CRYGA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRYGA sirna

siRNA to inhibit CRYGA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6724317
• NCBI GeneID: 12964
• NCBI Accession #: NP_031800.1
• NCBI GenBank Nucleotide #: NM_007774.3
• UniProt Accession #: P04345
• Molecular Weight: 21,149 Da
• NCBI Official Full Name: gamma-crystallin A
• NCBI Official Synonym Full Names: crystallin, gamma A
• NCBI Official Symbol: Cryga
• NCBI Official Synonym Symbols: Cryg-4; DGcry-4
• NCBI Protein Information: gamma-crystallin A
• UniProt Protein Name: Gamma-crystallin A
• Protein Family: Gamma-crystallin
• UniProt Gene Name: Cryga
• UniProt Entry Name: CRGA_MOUSE

NCBI Description

Three main families of major soluble proteins, the alpha, beta and gamma crystallins, are ubiquitously expressed in vertebrate lenses. This gene encodes a member of the gamma-crystallin family of proteins which may function as a structural component of the eye lens. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Five gamma-crystallin genes (gamma-A through gamma-E) are tandemly organized in a genomic segment as a gene cluster in the mouse. Another gamma-crystallin gene (gamma-F) is found some distance upstream of the cluster on the same chromosome. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008]

Uniprot Description

CRYGA: Crystallins are the dominant structural components of the vertebrate eye lens. Belongs to the beta/gamma-crystallin family.

Molecular Function: structural constituent of eye lens

Biological Process: lens development in camera-type eye; eye development