PEX1 sirna

PEX1 siRNA (Mouse)

Cat. Num. AAA828379

• Gene Names: Pex1; ZWS1; 5430414H02Rik; E330005K07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PEX1; PEX1 siRNA (Mouse); Peroxisome biogenesis factor 1; Peroxin-1; PEX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PEX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1284

• Applicable Applications for PEX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PEX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PEX1 sirna

siRNA to inhibit PEX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 658309686
• NCBI GeneID: 71382
• NCBI Accession #: NP_001280735.1
• NCBI GenBank Nucleotide #: NM_001293806.1
• UniProt Accession #: Q5BL07; Q3UEC7; Q9CU85
• Molecular Weight: 136,743 Da
• NCBI Official Full Name: peroxisome biogenesis factor 1 isoform 1
• NCBI Official Synonym Full Names: peroxisomal biogenesis factor 1
• NCBI Official Symbol: Pex1
• NCBI Official Synonym Symbols: ZWS1; 5430414H02Rik; E330005K07Rik
• NCBI Protein Information: peroxisome biogenesis factor 1
• UniProt Protein Name: Peroxisome biogenesis factor 1
• Protein Family: Peroxisomal ATPase
• UniProt Gene Name: Pex1
• UniProt Entry Name: PEX1_MOUSE

Uniprot Description

PEX1: Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes. Belongs to the AAA ATPase family. Interacts directly with PEX6. Interacts indirectly with PEX26, via its interaction with PEX6.

Protein type: Hydrolase

Cellular Component: peroxisomal membrane; intracellular membrane-bound organelle; membrane; cytoplasm; peroxisome; cytosol

Molecular Function: protein C-terminus binding; protein complex binding; nucleotide binding; ATPase activity, coupled; lipid binding; ATP binding

Biological Process: protein transport; transport; protein targeting to peroxisome; peroxisome organization and biogenesis; metabolic process; microtubule-based peroxisome localization; protein import into peroxisome matrix