SKAP1 sirna

SKAP1 siRNA (Rat)

Cat. Num. AAA828358

• Gene Names: Skap1; Scap1; Skap55
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SKAP1; SKAP1 siRNA (Rat); SCAP1; SKAP55; Src kinase-associated phosphoprotein 1; SKAP55 adapter protein; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP-55; pp55; SKAP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SKAP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 354

• Applicable Applications for SKAP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SKAP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SKAP1 sirna

siRNA to inhibit SKAP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 67514574
• NCBI GeneID: 286975
• NCBI Accession #: NP_775433.2
• NCBI GenBank Nucleotide #: NM_173311.2
• UniProt Accession #: Q4V7G1; Q8CHI6
• Molecular Weight: 40,903 Da
• NCBI Official Full Name: src kinase-associated phosphoprotein 1
• NCBI Official Synonym Full Names: src kinase associated phosphoprotein 1
• NCBI Official Symbol: Skap1
• NCBI Official Synonym Symbols: Scap1; Skap55
• NCBI Protein Information: src kinase-associated phosphoprotein 1
• UniProt Protein Name: Src kinase-associated phosphoprotein 1
• Protein Family: Src kinase-associated phosphoprotein
• UniProt Gene Name: Skap1
• UniProt Synonym Gene Names: Scap1; Skap55; SKAP-55
• UniProt Entry Name: SKAP1_RAT

NCBI Description

cytoplasmic phosphoprotein; member of the src family of kinase enzymes; may associate with mast cell immunoreceptor signal transducer (MIST) [RGD, Feb 2006]

Uniprot Description

SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; intercellular junction; cytosol; nucleus

Molecular Function: protein complex binding; SH2 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: positive regulation of cell adhesion; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway