BATF sirna

BATF siRNA (Mouse)

Cat. Num. AAA828142

• Gene Names: Batf; B-ATF; SFA-2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BATF; BATF siRNA (Mouse); Basic leucine zipper transcriptional factor ATF-like; B-cell-activating transcription factor; B-ATF; BATF sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BATF siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 125

• Applicable Applications for BATF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BATF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BATF sirna

siRNA to inhibit BATF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7949007
• NCBI GeneID: 53314
• NCBI Accession #: NP_058047.1
• NCBI GenBank Nucleotide #: NM_016767.2
• UniProt Accession #: O35284; A2RT86
• Molecular Weight: 14,065 Da
• NCBI Official Full Name: basic leucine zipper transcriptional factor ATF-like
• NCBI Official Synonym Full Names: basic leucine zipper transcription factor, ATF-like
• NCBI Official Symbol: Batf
• NCBI Official Synonym Symbols: B-ATF; SFA-2
• NCBI Protein Information: basic leucine zipper transcriptional factor ATF-like
• UniProt Protein Name: Basic leucine zipper transcriptional factor ATF-like
• Protein Family: Basic leucine zipper transcriptional factor
• UniProt Gene Name: Batf
• UniProt Synonym Gene Names: B-ATF
• UniProt Entry Name: BATF_MOUSE

Uniprot Description

BATF: a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. Functions as a negative regulator of AP-1 mediated transcription by binding to Jun proteins. Jun/B-ATF heterodimers bind DNA preferentially at the 12-O-tetradecanoylphorbol-13- acetate response element (TRE) and weaker at the cAMP responsive region (CRE), but are transcriptionally inert.

Protein type: DNA-binding; Transcription factor

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator; transcription, DNA-dependent; isotype switching; cytokine production; lymphoid progenitor cell differentiation; myeloid dendritic cell differentiation; defense response to protozoan; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; cell differentiation; response to DNA damage stimulus