ATG10 sirna

ATG10 siRNA (Mouse)

Cat. Num. AAA828044

• Gene Names: Atg10; APG10; Agp10; Apg10l; Apg10p; Atg10l; AI852123; 5330424L23Rik; 5430428K15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATG10; ATG10 siRNA (Mouse); APG10L; Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10; APG10-like; mAPG10; ATG10 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATG10 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 215

• Applicable Applications for ATG10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATG10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATG10 sirna

siRNA to inhibit ATG10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114158679
• NCBI GeneID: 66795
• NCBI Accession #: NP_080046.3
• NCBI GenBank Nucleotide #: NM_025770.3
• UniProt Accession #: Q8R1P4; Q8BPA9; Q9D3J7
• Molecular Weight: 24,577 Da
• NCBI Official Full Name: ubiquitin-like-conjugating enzyme ATG10
• NCBI Official Synonym Full Names: autophagy related 10
• NCBI Official Symbol: Atg10
• NCBI Official Synonym Symbols: APG10; Agp10; Apg10l; Apg10p; Atg10l; AI852123; 5330424L23Rik; 5430428K15Rik
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG10
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG10
• Protein Family: Ubiquitin-like-conjugating enzyme
• UniProt Gene Name: Atg10
• UniProt Synonym Gene Names: Apg10l; APG10-like; mAPG10
• UniProt Entry Name: ATG10_MOUSE

Uniprot Description

ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Autophagy

Cellular Component: cytoplasm; intracellular

Molecular Function: protein binding; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; APG12 conjugating enzyme activity; ligase activity

Biological Process: protein transport; protein modification by small protein conjugation; transport; positive regulation of protein modification process; protein amino acid lipidation; autophagy