FLVCR1 sirna

FLVCR1 siRNA (Mouse)

Cat. Num. AAA828014

• Gene Names: Mfsd7b; FLVCR; Flvcr1; 9630055N22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FLVCR1; FLVCR1 siRNA (Mouse); MFSD7B; Feline leukemia virus subgroup C receptor-related protein 1; Feline leukemia virus subgroup C receptor; Major facilitator superfamily domain containing 7B; Mfsd7b; FLVCR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FLVCR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 279

• Applicable Applications for FLVCR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FLVCR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FLVCR1 sirna

siRNA to inhibit FLVCR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124486925
• NCBI GeneID: 226844
• NCBI Accession #: NP_001074728.1
• NCBI GenBank Nucleotide #: NM_001081259.1
• UniProt Accession #: B2RXV4
• Molecular Weight: 30,838 Da
• NCBI Official Full Name: feline leukemia virus subgroup C receptor-related protein 1
• NCBI Official Synonym Full Names: major facilitator superfamily domain containing 7B
• NCBI Official Symbol: Mfsd7b
• NCBI Official Synonym Symbols: FLVCR; Flvcr1; 9630055N22Rik
• NCBI Protein Information: feline leukemia virus subgroup C receptor-related protein 1
• UniProt Protein Name: Feline leukemia virus subgroup C receptor-related protein 1
• Protein Family: Feline leukemia virus subgroup C receptor-related protein
• UniProt Gene Name: Flvcr1
• UniProt Synonym Gene Names: Mfsd7b; Feline leukemia virus subgroup C receptor; Mfsd7b
• UniProt Entry Name: FLVC1_MOUSE

Uniprot Description

FLVCR1: Heme transporter that exports cytoplasmic heme. It can also export coproporphyrin and protoporphyrin IX, which are both intermediate products in the heme biosynthetic pathway. Does not export bilirubin. Heme export depends on the presence of HPX and may be required to protect developing erythroid cells from heme toxicity. Heme export also provides protection from heme or ferrous iron toxicities in liver and brain. Causes susceptibility to FeLV-C in vitro. Defects in FLVCR1 are the cause of posterior column ataxia with retinitis pigmentosa (PCARP). A neurodegenerative syndrome beginning in infancy with areflexia and retinitis pigmentosa. Nyctalopia (night blindness) and peripheral visual field loss are usually evident during late childhood or teenage years, with subsequent progressive constriction of the visual fields and loss of central retinal function over time. A sensory ataxia caused by degeneration of the posterior columns of the spinal cord results in a loss of proprioceptive sensation that is clinically evident in the second decade of life and gradually progresses. Scoliosis, camptodactyly, achalasia, gastrointestinal dysmotility, and a sensory peripheral neuropathy are variable features of the disease. Affected individuals have no clinical or radiological evidence of cerebral or cerebellar involvement. Defective neuronal heme transmembrane export due to FLVCR1 mutations may abrogate the neuroprotective effects of neuroglobin and initiate an apoptotic cascade that results in the selective degeneration of photoreceptors in the neurosensory retina and sensory neurons in the posterior spinal cord. Belongs to the major facilitator superfamily. Feline leukemia virus subgroup C receptor (TC 2.A.1.28.1) family.

Protein type: Transporter; Transporter, ion channel; Transporter, iron; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: membrane; mitochondrion; integral to membrane; plasma membrane

Molecular Function: heme transporter activity

Biological Process: spleen development; blood vessel development; heme transport; erythrocyte maturation; in utero embryonic development; multicellular organism growth; regulation of organ growth; limb morphogenesis; mitochondrial transport; embryonic skeletal morphogenesis; transport; erythrocyte differentiation; embryonic digit morphogenesis; transmembrane transport