ACLY sirna

ACLY siRNA (Human)

Cat. Num. AAA827995

• Gene Names: ACLY; ACL; ATPCL; CLATP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACLY; ACLY siRNA (Human); ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme; ACLY sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ACLY siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 830

• Applicable Applications for ACLY sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACLY gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACLY sirna

siRNA to inhibit ACLY expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38569421
• NCBI GeneID: 47
• NCBI Accession #: NP_001087.2
• NCBI GenBank Nucleotide #: NM_001096.2
• UniProt Accession #: P53396; Q13037; Q9BRL0; B4DIM0; B4E3P0
• Molecular Weight: 91,099 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 1
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: ACLY
• NCBI Official Synonym Symbols: ACL; ATPCL; CLATP
• NCBI Protein Information: ATP-citrate synthase
• UniProt Protein Name: ATP-citrate synthase
• Protein Family: ATP-citrate synthase
• UniProt Gene Name: ACLY
• UniProt Synonym Gene Names: ACL
• UniProt Entry Name: ACLY_HUMAN

NCBI Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.3.3.8; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; mitochondrion; membrane; citrate lyase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; ATP citrate synthase activity; metal ion binding; cofactor binding; succinate-CoA ligase (ADP-forming) activity; ATP binding

Biological Process: coenzyme A metabolic process; lipid biosynthetic process; positive regulation of cellular metabolic process; energy reserve metabolic process; citrate metabolic process; cellular carbohydrate metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process