RING1 sirna

RING1 siRNA (Rat)

Cat. Num. AAA827735

• Gene Names: Ring1; Ring1A
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RING1; RING1 siRNA (Rat); RNF1; E3 ubiquitin-protein ligase RING1; Polycomb complex protein RING1; RING finger protein 1; RING1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: RING1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 377

• Applicable Applications for RING1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RING1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RING1 sirna

siRNA to inhibit RING1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 402766203
• NCBI GeneID: 309626
• NCBI Accession #: NP_997714.2
• NCBI GenBank Nucleotide #: NM_212549.2
• UniProt Accession #: Q6MGB6; Q4KMC1; Q63510
• Molecular Weight: 39,377 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RING1
• NCBI Official Synonym Full Names: ring finger protein 1
• NCBI Official Symbol: Ring1
• NCBI Official Synonym Symbols: Ring1A
• NCBI Protein Information: E3 ubiquitin-protein ligase RING1
• UniProt Protein Name: E3 ubiquitin-protein ligase RING1
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: Ring1
• UniProt Synonym Gene Names: Rnf1
• UniProt Entry Name: RING1_RAT

NCBI Description

maps within the major histocompatibility complex RT1 region [RGD, Feb 2006]

Uniprot Description

RING1: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity (By similarity)

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Transcription factor; Ligase; EC 6.3.2.-

Cellular Component: nucleoplasm; nuclear body; sex chromatin; cytoplasm; nuclear speck; PcG protein complex; nucleus; ubiquitin ligase complex

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: anterior/posterior pattern formation; camera-type eye morphogenesis; transcription, DNA-dependent; regulation of catalytic activity; histone ubiquitination; negative regulation of transcription, DNA-dependent