ACHE sirna

ACHE siRNA (Mouse)

Cat. Num. AAA827538

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACHE; ACHE siRNA (Mouse); Acetylcholinesterase; AChE; ACHE sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACHE siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 614

• Applicable Applications for ACHE sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACHE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACHE sirna

siRNA to inhibit ACHE expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 586597902
• NCBI GeneID: 11423
• NCBI Accession #: NP_001276939.1
• NCBI GenBank Nucleotide #: NM_001290010.1
• UniProt Accession #: P21836
• Molecular Weight: – Da
• NCBI Official Full Name: acetylcholinesterase
• NCBI Official Synonym Full Names: acetylcholinesterase
• NCBI Official Symbol: Ache
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Achelase
• UniProt Gene Name: Ache
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_MOUSE

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Hydrolase; Membrane protein, GPI anchor; Lipid Metabolism - glycerophospholipid

Cellular Component: Golgi apparatus; extracellular space; neuron projection; cell surface; rough endoplasmic reticulum; endoplasmic reticulum lumen; dendrite; extracellular region; nuclear envelope; presynaptic membrane; postsynaptic membrane; membrane; cell soma; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; basal lamina; synapse; cell junction; neuromuscular junction

Molecular Function: collagen binding; choline binding; serine hydrolase activity; protein binding; protein homodimerization activity; protein self-association; cholinesterase activity; hydrolase activity; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: neurotransmitter catabolic process; choline metabolic process; synaptogenesis; retina development in camera-type eye; acetylcholine metabolic process; neurotransmitter receptor biosynthetic process; acetylcholine catabolic process; receptor internalization; regulation of receptor recycling; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; cell adhesion; protein tetramerization