SHARPIN sirna

SHARPIN siRNA (Mouse)

Cat. Num. AAA827085

• Gene Names: Sharpin; cpdm; SIPL1; RBCKL1; AW121341; 0610041B22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SHARPIN; SHARPIN siRNA (Mouse); CPDM; SIPL1; Sharpin; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; mSIPL1; SHARPIN sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SHARPIN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 380

• Applicable Applications for SHARPIN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SHARPIN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SHARPIN sirna

siRNA to inhibit SHARPIN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 165377121
• NCBI GeneID: 106025
• NCBI Accession #: NP_079616.2
• NCBI GenBank Nucleotide #: NM_025340.2
• UniProt Accession #: Q91WA6; Q7TNT3; Q8CHL3
• Molecular Weight: 31,864 Da
• NCBI Official Full Name: sharpin
• NCBI Official Synonym Full Names: SHANK-associated RH domain interacting protein
• NCBI Official Symbol: Sharpin
• NCBI Official Synonym Symbols: cpdm; SIPL1; RBCKL1; AW121341; 0610041B22Rik
• NCBI Protein Information: sharpin
• UniProt Protein Name: Sharpin
• Protein Family: Sharpin
• UniProt Gene Name: Sharpin
• UniProt Synonym Gene Names: Cpdm; Sipl1; mSIPL1
• UniProt Entry Name: SHRPN_MOUSE

Uniprot Description

SHARPIN: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Self-associates. Interacts with SHANK1, EYA1 and EYA2. Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation- dependent manner. Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Cellular Component: nucleoplasm; postsynaptic density; cytoplasm; dendrite; synapse; cytosol; nucleus; cell junction

Molecular Function: identical protein binding; protein binding; zinc ion binding; metal ion binding; protein complex binding; polyubiquitin binding

Biological Process: mitochondrion organization and biogenesis; epidermis development; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptotic nuclear changes; negative regulation of inflammatory response; keratinization; protein homooligomerization