PGGT1B sirna

PGGT1B siRNA (Mouse)

Cat. Num. AAA826875

• Gene Names: Pggt1b; BGG1; GGT1; AI451237; AI551093; 2610100E13; 2010207C17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PGGT1B; PGGT1B siRNA (Mouse); Geranylgeranyl transferase type-1 subunit beta; Geranylgeranyl transferase type I subunit beta; GGTase-I-beta; Type I protein geranyl-geranyltransferase subunit beta; PGGT1B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PGGT1B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 377

• Applicable Applications for PGGT1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PGGT1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PGGT1B sirna

siRNA to inhibit PGGT1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27369904
• NCBI GeneID: 225467
• NCBI Accession #: NP_766215.1
• NCBI GenBank Nucleotide #: NM_172627.3
• UniProt Accession #: Q8BUY9
• Molecular Weight: 42,354 Da
• NCBI Official Full Name: geranylgeranyl transferase type-1 subunit beta
• NCBI Official Synonym Full Names: protein geranylgeranyltransferase type I, beta subunit
• NCBI Official Symbol: Pggt1b
• NCBI Official Synonym Symbols: BGG1; GGT1; AI451237; AI551093; 2610100E13; 2010207C17Rik
• NCBI Protein Information: geranylgeranyl transferase type-1 subunit beta
• UniProt Protein Name: Geranylgeranyl transferase type-1 subunit beta
• UniProt Gene Name: Pggt1b
• UniProt Synonym Gene Names: GGTase-I-beta
• UniProt Entry Name: PGTB1_MOUSE

Uniprot Description

PGGT1B: Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding. Belongs to the protein prenyltransferase subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.5.1.59

Molecular Function: transferase activity; protein geranylgeranyltransferase activity; isoprenoid binding; zinc ion binding; metal ion binding; CAAX-protein geranylgeranyltransferase activity; drug binding; peptide binding; catalytic activity; prenyltransferase activity

Biological Process: protein geranylgeranylation; negative regulation of nitric-oxide synthase biosynthetic process; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of cell cycle