TNNI3K sirna

TNNI3K siRNA (Human)

Cat. Num. AAA826860

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TNNI3K; TNNI3K siRNA (Human); CARK; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase; TNNI3K sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TNNI3K siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 856

• Applicable Applications for TNNI3K sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TNNI3K gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TNNI3K sirna

siRNA to inhibit TNNI3K expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 510785758
• NCBI GeneID: 100526835
• NCBI Accession #: NP_001186256.2
• NCBI GenBank Nucleotide #: NM_001199327.1
• UniProt Accession #: Q59H18; Q17RN0; Q49AR1; Q6MZS9; Q9Y2V6
• Molecular Weight: 94,517 Da
• NCBI Official Full Name: FPGT-TNNI3K fusion protein isoform b
• NCBI Official Synonym Full Names: FPGT-TNNI3K readthrough
• NCBI Official Symbol: FPGT-TNNI3K
• NCBI Protein Information: FPGT-TNNI3K fusion protein
• UniProt Protein Name: Serine/threonine-protein kinase TNNI3K
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: TNNI3K
• UniProt Entry Name: TNI3K_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010]

Uniprot Description

TNNI3K: May play a role in cardiac physiology. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; HH498 subfamily

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein serine/threonine kinase activity; protein binding; troponin I binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: regulation of heart rate; protein amino acid phosphorylation

Disease: Cardiac Conduction Disease With Or Without Dilated Cardiomyopathy