SEMA4D recombinant protein
Recombinant Mouse SEMA4D Protein
Gene Names
Sema4d; CD100; Semaj; Semcl2; coll-4; Semacl2
Reactivity
Mouse
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Synonyms
SEMA4D; Recombinant Mouse SEMA4D Protein; C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; GR3; SEMA4D recombinant protein
Host
Human cells
Reactivity
Mouse
Purity/Purification
Greater than 95% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Sequence
FAPVPRLTWE HGEVGLVQFH KPGIFNYSAL LMSEDKDTLY VGAREAVFAV NALNISEKQH EVYWKVSEDK KSKCAEKGKS KQTECLNYIR VLQPLSSTSL YVCGTNAFQP TCDHLNLTSF KFLGKSEDGK GRCPFDPAHS YTSVMVGGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIQKSPDG PEGEDDKVYF FFTEVSVEYE FVFKLMIPRV ARVCKGDQGG LRTLQKKWTS FLKARLICSK PDSGLVFNIL QDVFVLRAPG LKEPVFYAVF TPQLNNVGLS AVCAYTLATV EAVFSRGKYM QSATVEQSHT KWVRYNGPVP TPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPKL IKKDVNYTQI VVDRTQALDG TFYDVMFIST DRGALHKAVI LTKEVHVIEE TQLFRDSEPV LTLLLSSKKG RKFVYAGSNS GVVQAPLAFC EKHGSCEDCV LARDPYCAWS PAIKACVTLH QEEASSRGWI QDMSGDTSSC LDKSKESFNQ HFFKHGGTAE LKCFQKSNLA RVVWKFQNGE LKAASPKYGF VGRKHLLIFN LSDGDSGVYQ CLSEERVRNK TVSQLLAKHV LEVKMVPRTP PSPTSEDAQT EGSKITSKMP VASTQGSSPP TPALWATSPR AATLPPKSSS GTSCEPKMVD DIEGRMDEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
Sequence Length
861
Applicable Applications for SEMA4D recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for SEMA4D recombinant protein
Recombinant Mouse SEMA4D Protein is produced by our mammalian expression system and the target gene encoding Phe24-Met711 is expressed with a Fc tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95,640 Da
NCBI Official Full Name
semaphorin-4D
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
Sema4d
NCBI Official Synonym Symbols
CD100; Semaj; Semcl2; coll-4; Semacl2
NCBI Protein Information
semaphorin-4D
UniProt Protein Name
Semaphorin-4D
Protein Family
UniProt Gene Name
Sema4d
UniProt Synonym Gene Names
Semacl2; Semaj; Sema J
UniProt Entry Name
SEM4D_MOUSE
Uniprot Description
SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Protein type: Membrane protein, integral
Cellular Component: extracellular space; integral to plasma membrane; membrane; plasma membrane
Molecular Function: chemorepellent activity; protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity
Biological Process: negative chemotaxis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; positive regulation of axonogenesis; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; regulation of cell projection organization and biogenesis; regulation of cell shape; regulation of dendrite morphogenesis
Research Articles on SEMA4D
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Product Notes
The SEMA4D sema4d (Catalog #AAA8249751) is a Recombinant Protein produced from Human cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Mouse SEMA4D Protein reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the SEMA4D sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FAPVPRLTWE HGEVGLVQFH KPGIFNYSAL LMSEDKDTLY VGAREAVFAV NALNISEKQH EVYWKVSEDK KSKCAEKGKS KQTECLNYIR VLQPLSSTSL YVCGTNAFQP TCDHLNLTSF KFLGKSEDGK GRCPFDPAHS YTSVMVGGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIQKSPDG PEGEDDKVYF FFTEVSVEYE FVFKLMIPRV ARVCKGDQGG LRTLQKKWTS FLKARLICSK PDSGLVFNIL QDVFVLRAPG LKEPVFYAVF TPQLNNVGLS AVCAYTLATV EAVFSRGKYM QSATVEQSHT KWVRYNGPVP TPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPKL IKKDVNYTQI VVDRTQALDG TFYDVMFIST DRGALHKAVI LTKEVHVIEE TQLFRDSEPV LTLLLSSKKG RKFVYAGSNS GVVQAPLAFC EKHGSCEDCV LARDPYCAWS PAIKACVTLH QEEASSRGWI QDMSGDTSSC LDKSKESFNQ HFFKHGGTAE LKCFQKSNLA RVVWKFQNGE LKAASPKYGF VGRKHLLIFN LSDGDSGVYQ CLSEERVRNK TVSQLLAKHV LEVKMVPRTP PSPTSEDAQT EGSKITSKMP VASTQGSSPP TPALWATSPR AATLPPKSSS GTSCEPKMVD DIEGRMDEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK. It is sometimes possible for the material contained within the vial of "SEMA4D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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