MIF recombinant protein

Recombinant Mouse MIF Protein

Cat. Num. AAA8249451

• Gene Names: Mif; GIF; Glif
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: MIF; Recombinant Mouse MIF Protein; GLIF; MMIF; Macrophage migration inhibitory factor; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase; MIF recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: PMFIVNTNVP RASVPEGFLS ELTQQLAQAT GKPAQYIAVH VVPDQLMTFS GTNDPCALCS LHSIGKIGGA QNRNYSKLLC GLLSDRLHIS PDRVYINYYD MNAANVGWNG STFALEHHHH HH
• Sequence Length: 115

• Applicable Applications for MIF recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for MIF recombinant protein

Recombinant Mouse MIF Protein is produced by our E Coli expression system and the target gene encoding Pro2-Ala115 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6754696
• NCBI GeneID: 17319
• NCBI Accession #: NP_034928.1
• NCBI GenBank Nucleotide #: NM_010798.2
• UniProt Accession #: P34884
• Molecular Weight: 12,504 Da
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• NCBI Official Symbol: Mif
• NCBI Official Synonym Symbols: GIF; Glif
• NCBI Protein Information: macrophage migration inhibitory factor
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: MIF-like protein
• UniProt Gene Name: Mif
• UniProt Synonym Gene Names: MIF; DER6; GIF
• UniProt Entry Name: MIF_MOUSE

Uniprot Description

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Apoptosis; Cell development/differentiation; Cytokine; EC 5.3.2.1; EC 5.3.3.12; Isomerase

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; nucleus; vesicle

Molecular Function: chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein complex binding; receptor binding

Biological Process: brain renin-angiotensin system; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; drinking behavior; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive regulation of acute inflammatory response; positive regulation of axon regeneration; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of glucose import; positive regulation of glycolysis; positive regulation of immune response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of potassium ion transport; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell migration; prostaglandin biosynthetic process; regulation of cell proliferation; spermatogenesis