GABARAPL1 recombinant protein

Recombinant Human GABARAPL1 Protein

Cat. Num. AAA8249079

• Gene Names: GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: GABARAPL1; Recombinant Human GABARAPL1 Protein; GEC1; Gamma-aminobutyric acid receptor-associated protein-like 1; Early estrogen-regulated protein; GABA(A receptor-associated protein-like 1; Glandular epithelial cell protein 1; GEC-1; GABARAPL1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: MGSSHHHHHH SSGLVPRGSH MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE EDYFLYVAYS DESVYGK
• Sequence Length: 117

• Applicable Applications for GABARAPL1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for GABARAPL1 recombinant protein

Recombinant Human GABARAPL1 Protein is produced by our E Coli expression system and the target gene encoding Met1-Lys117 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13899219
• NCBI GeneID: 23710
• NCBI Accession #: NP_113600.1
• NCBI GenBank Nucleotide #: NM_031412.2
• UniProt Accession #: Q9H0R8; Q6FIE6; B4E0Y7
• Molecular Weight: 17,008 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein-like 1
• NCBI Official Synonym Full Names: GABA type A receptor associated protein like 1
• NCBI Official Symbol: GABARAPL1
• NCBI Official Synonym Symbols: ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 1
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 1
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAPL1
• UniProt Synonym Gene Names: GEC1; GEC-1
• UniProt Entry Name: GBRL1_HUMAN

Uniprot Description

GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1.

Protein type: Adaptor/scaffold; Autophagy; Microtubule-binding; Ubiquitin-like modifier; Vesicle

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: autophagic vacuole; cytosol; intracellular; mitochondrion

Molecular Function: beta-tubulin binding; GABA receptor binding; protein binding; Tat protein binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation