FZD7 recombinant protein

Recombinant Human FZD7 Protein

Cat. Num. AAA8249075

• Gene Names: FZD7; FzE3
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: FZD7; Recombinant Human FZD7 Protein; Frizzled-7; Fz-7; hFz7; FzE3; FZD7 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: QPYHGEKGIS VPDHGFCQPI SIPLCTDIAY NQTILPNLLG HTNQEDAGLE VHQFYPLVKV QCSPELRFFL CSMYAPVCTV LDQAIPPCRS LCERARQGCE ALMNKFGFQW PERLRCENFP VHGAGEICVG QNTSDGSGGP GGGPTAYPTA PYLVDHHHHH H
• Sequence Length: 574

• Applicable Applications for FZD7 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for FZD7 recombinant protein

Recombinant Human FZD7 Protein is produced by our Mammalian expression system and the target gene encoding M1-L185 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4503833
• NCBI GeneID: 8324
• NCBI Accession #: NP_003498.1
• NCBI GenBank Nucleotide #: NM_003507.1
• UniProt Accession #: O75084; O94816; Q53S59; Q96B74
• Molecular Weight: 63,620 Da
• NCBI Official Full Name: frizzled-7
• NCBI Official Synonym Full Names: frizzled class receptor 7
• NCBI Official Symbol: FZD7
• NCBI Official Synonym Symbols: FzE3
• NCBI Protein Information: frizzled-7
• UniProt Protein Name: Frizzled-7
• Protein Family: Frizzled
• UniProt Gene Name: FZD7
• UniProt Synonym Gene Names: Fz-7; hFz7
• UniProt Entry Name: FZD7_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD7: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to membrane; plasma membrane; recycling endosome membrane

Molecular Function: frizzled binding; G-protein coupled receptor activity; PDZ domain binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: negative regulation of ectodermal cell fate specification; neuron differentiation; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of JNK cascade; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; stem cell maintenance; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway