CTSB recombinant protein

Recombinant Mouse CTSB Protein

Cat. Num. AAA8248879

• Gene Names: Ctsb; CB
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CTSB; Recombinant Mouse CTSB Protein; CPSB; Cathepsin B; APP secretase; APPS; Cathepsin B1; CTSB recombinant protein

• Host: Human cells
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFVDHHHHHH
• Sequence Length: 339

• Applicable Applications for CTSB recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CTSB recombinant protein

Recombinant Mouse CTSB Protein is produced by our mammalian expression system and the target gene encoding His18-Phe339 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6681079
• NCBI GeneID: 13030
• NCBI Accession #: NP_031824.1
• NCBI GenBank Nucleotide #: NM_007798.3
• UniProt Accession #: P10605; Q3UDW7
• Molecular Weight: 37,280 Da
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: Ctsb
• NCBI Official Synonym Symbols: CB
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsb
• UniProt Entry Name: CATB_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Autophagy; EC 3.4.22.1; Motility/polarity/chemotaxis; Protease

Cellular Component: apical plasma membrane; caveola; cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; intracellular membrane-bound organelle; lysosome; mitochondrion; nucleolus; perinuclear region of cytoplasm; sarcolemma

Molecular Function: collagen binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; kininogen binding; peptidase activity; peptide binding; protein complex binding; protein self-association; proteoglycan binding

Biological Process: collagen catabolic process; decidualization; entry of virus into host cell; proteolysis; proteolysis involved in cellular protein catabolic process