CPA2 recombinant protein

Recombinant Mouse CPA2 Protein

Cat. Num. AAA8248820

• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CPA2; Recombinant Mouse CPA2 Protein; Carboxypeptidase A2; CPA2 recombinant protein

• Host: Human cells
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Sequence: QETFVGDQVL EVIPNDEEQI KTLLQLEAEE HLELDFWKSP SVPRQTVHVR VPFASIQDVK VFLESQGITY SIMIEDVQVL LDQEREEMLF NQQRERGTNF NFGAYHTLEE IYQEMDNLVA ENPGLVSKVN IGSSFENRPM NVLKFSTGGD KPAIWLDAGI HAREWVTQAT ALWTANKIAS DYGTDPAITS LLNTLDVFLL PVTNPDGYVF SQTSNRMWRK TRSKRSGSFC VGVDPNRNWD ANFGGPGASS NPCSDSYHGP SPNSEVEVKS IVDFIKSHGK VKAFITLHSY SQLLMFPYGY KCAKPDDFNE LDEVAQRAAQ SLKRLHGTSY KVGPICSVIY QASGGSIDWA YDLGIKYSFA FELRDTGYYG FLLPAKQILP TAEETWLGLK TIMEHVRDHP YVDHHHHHH
• Sequence Length: 417

• Applicable Applications for CPA2 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CPA2 recombinant protein

Recombinant Mouse CPA2 Protein is produced by our mammalian expression system and the target gene encoding Gln17-Tyr417 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 67010039
• NCBI GeneID: 232680
• NCBI Accession #: NP_001019869.1
• NCBI GenBank Nucleotide #: NM_001024698.3
• UniProt Accession #: Q504N0
• Molecular Weight: 47,057 Da
• NCBI Official Full Name: carboxypeptidase A2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase A2, pancreatic
• NCBI Official Symbol: Cpa2
• NCBI Protein Information: carboxypeptidase A2
• UniProt Protein Name: Carboxypeptidase A2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: Cpa2
• UniProt Entry Name: CBPA2_MOUSE

NCBI Description

This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein undergoes proteolytic processing that removes the N-terminal activation peptide to generate a functional enzyme. This gene is expressed by the pancreatic exocrine cells which secrete the enzyme during digestion. This gene is located in a cluster of carboxypeptidase genes on chromosome 6. [provided by RefSeq, Jul 2016]

Uniprot Description

CPA2: Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq, Dec 2008]

Protein type: EC 3.4.17.15; Protease; Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space

Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity; zinc ion binding