BNIP3 recombinant protein

Recombinant Human BNIP3 Protein

Cat. Num. AAA8248616

• Gene Names: BNIP3; NIP3
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: BNIP3; Recombinant Human BNIP3 Protein; NIP3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: MNHKVHHHHH HMSQNGAPGM QEESLQGSWV ELHFSNNGNG GSVPASVSIY NGDMEKILLD AQHESGRSSS KSSHCDSPPR SQTPQDTNRA SETDTHSIGE KNSSQSEEDD IERRKEVESI LKKNSDWIWD WSSRPENIPP KEFLFKHPKR TATLSMRNTS VMKKGGIFSA EFLKVFLLSR PAV
• Sequence Length: 259

• Applicable Applications for BNIP3 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for BNIP3 recombinant protein

Recombinant Human BNIP3 Protein is produced by our E Coli expression system and the target gene encoding Met1-Leu166 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 734703979
• NCBI GeneID: 664
• NCBI Accession #: NP_004043.3
• NCBI GenBank Nucleotide #: NM_004052.3
• UniProt Accession #: Q12983; O14620; Q96GP0
• Molecular Weight: 21,541 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• NCBI Official Synonym Full Names: BCL2 interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BNIP3
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cytoplasm; dendrite; endoplasmic reticulum; integral to mitochondrial outer membrane; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; nuclear envelope; nucleoplasm; nucleus

Molecular Function: GTPase binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: apoptosis; cell death; defense response to virus; induction of apoptosis by granzyme; mitochondrial fragmentation during apoptosis; negative regulation of apoptosis; negative regulation of membrane potential; neuron apoptosis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of macroautophagy; positive regulation of programmed cell death; positive regulation of protein complex disassembly; regulation of mitochondrial membrane permeability; response to hypoxia