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BDH2 recombinant protein

Recombinant Human BDH2 Protein

Gene Names
BDH2; DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
Reactivity
Human
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Synonyms
BDH2; Recombinant Human BDH2 Protein; DHRS6; 3-hydroxybutyrate dehydrogenase type 2; Dehydrogenase/reductase SDR family member 6; Oxidoreductase UCPA; R-beta-hydroxybutyrate dehydrogenase; BDH2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Reactivity
Human
Purity/Purification
Greater than 95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Sequence
MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAS EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL
Sequence Length
245
Applicable Applications for BDH2 recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for BDH2 recombinant protein
Recombinant Human BDH2 Protein is produced by our E Coli expression system and the target gene encoding Met1-Leu245 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,626 Da
NCBI Official Full Name
3-hydroxybutyrate dehydrogenase type 2
NCBI Official Synonym Full Names
3-hydroxybutyrate dehydrogenase 2
NCBI Official Symbol
BDH2
NCBI Official Synonym Symbols
DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
NCBI Protein Information
3-hydroxybutyrate dehydrogenase type 2
UniProt Protein Name
3-hydroxybutyrate dehydrogenase type 2
UniProt Gene Name
BDH2
UniProt Synonym Gene Names
DHRS6; SDR15C1
UniProt Entry Name
BDH2_HUMAN

Uniprot Description

DHRS6: Dehydrogenase that mediates the formation of 2,5- dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - butanoate; EC 1.1.1.30; Lipid Metabolism - synthesis and degradation of ketone bodies; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytoplasm; cytosol; mitochondrion

Molecular Function: 3-hydroxybutyrate dehydrogenase activity; NAD binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor

Biological Process: epithelial cell differentiation; fatty acid beta-oxidation; heme metabolic process; iron ion homeostasis; ketone body biosynthetic process; siderophore biosynthetic process

Research Articles on BDH2

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Product Notes

The BDH2 bdh2 (Catalog #AAA8248601) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human BDH2 Protein reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BDH2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the BDH2 bdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAS EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL. It is sometimes possible for the material contained within the vial of "BDH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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