Western Blot (WB)
(Western blot analysis of Thymidine Phosphorylase expression in A549 (A), THP1 (B) whole cell lysates.)
Rabbit Thymidine Phosphorylase Polyclonal Antibody | anti-TYMP antibody
Anti-Thymidine Phosphorylase Antibody
Western Blot (WB)
(Western blot analysis of Thymidine Phosphorylase expression in A549 (A), THP1 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Thymidine Phosphorylase expression in A549 (A), THP1 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Thymidine Phosphorylase staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Thymidine Phosphorylase staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
Uniprot Description
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: EC 2.4.2.4; Cytokine; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis; Phosphorylase; Transferase; DNA replication
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: phosphorylase activity; platelet-derived growth factor receptor binding; transferase activity, transferring pentosyl groups
Biological Process: DNA replication; mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine nucleoside salvage; pyrimidine nucleotide metabolic process
Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
Research Articles on TYMP
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Product Notes
The TYMP tymp (Catalog #AAA8247716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Thymidine Phosphorylase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Thymidine Phosphorylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1/500 - 1/2000, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200. Researchers should empirically determine the suitability of the TYMP tymp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymidine Phosphorylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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