Western Blot (WB)
(Western blot analysis of RPLP1 expression in SW480 (A), mouse spleen (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates.)
Rabbit anti-Human, Rat RPLP1 Polyclonal Antibody | anti-RPLP1 antibody
Anti-RPLP1 Antibody
Western Blot (WB)
(Western blot analysis of RPLP1 expression in SW480 (A), mouse spleen (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RPLP1 expression in SW480 (A), mouse spleen (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RPLP1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RPLP1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of RPLP1 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of RPLP1 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Uniprot Description
RPLP1: Plays an important role in the elongation step of protein synthesis. Belongs to the ribosomal protein L12P family.
Protein type: Translation; Ribosomal
Chromosomal Location of Human Ortholog: 15q22
Cellular Component: cytoplasm; cytosol; focal adhesion
Molecular Function: protein binding; protein kinase activator activity; structural constituent of ribosome
Biological Process: mRNA catabolic process, nonsense-mediated decay; positive regulation of protein kinase activity; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
Research Articles on RPLP1
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Product Notes
The RPLP1 rplp1 (Catalog #AAA8247631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPLP1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPLP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1/500 - 1/2000, Immunohistochemistry: 1/50 - 1/200, Immunofluorescence/Immunocytochemistry: 1/50 - 1/100. Researchers should empirically determine the suitability of the RPLP1 rplp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPLP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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