Rabbit RNGTT Polyclonal Antibody | anti-RNGTT antibody

Anti-RNGTT Antibody

Cat. Num. AAA8245861

• Gene Names: RNGTT; HCE; HCE1; hCAP; CAP1A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: RNGTT; Polyclonal Antibody; Anti-RNGTT Antibody; CAP1A; mRNA-capping enzyme; HCAP1; HCE; anti-RNGTT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of RNGTT protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 541

• Applicable Applications for anti-RNGTT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200
• Immunogen: Recombinant full length protein of human RNGTT
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of RNGTT expression in Jurkat (A), Hela (B), mouse spleen (C), rat kidney (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RNGTT staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of RNGTT staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Related Product Information for anti-RNGTT antibody

Rabbit polyclonal antibody to RNGTT

NCBI and Uniprot Product Information

• NCBI GI #: 556695469
• NCBI GeneID: 8732
• NCBI Accession #: NP_001273355.1
• NCBI GenBank Nucleotide #: NM_001286426.1
• UniProt Accession #: O60942; O43483; O60257; O60351; Q5TCW8; Q8WUM8; E1P513; E1P514
• NCBI Official Full Name: mRNA-capping enzyme isoform b
• NCBI Official Synonym Full Names: RNA guanylyltransferase and 5'-phosphatase
• NCBI Official Symbol: RNGTT
• NCBI Official Synonym Symbols: HCE; HCE1; hCAP; CAP1A
• NCBI Protein Information: mRNA-capping enzyme
• UniProt Protein Name: mRNA-capping enzyme
• UniProt Gene Name: RNGTT
• UniProt Synonym Gene Names: CAP1A; TPase; GTase
• UniProt Entry Name: MCE1_HUMAN

Uniprot Description

RNGTT: a bifunctional mRNA capping enzyme with RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Four alternatively spliced human isoforms have been described. Isoform 1 and isoform 4 are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. Isoforms 2, 3 and 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTPase domain.

Protein type: Motility/polarity/chemotaxis; Phosphatase (non-protein); Transferase; EC 3.1.3.33; EC 2.7.7.50

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: nucleoplasm; nucleus

Molecular Function: GTP binding; mRNA guanylyltransferase activity; polynucleotide 5'-phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; RNA guanylyltransferase activity; triphosphatase activity

Biological Process: mRNA capping; RNA processing; transcription from RNA polymerase II promoter; viral reproduction