Western Blot (WB)
(Western blot analysis of PSAT1 expression in Hela (A), SKOV3 (B), mouse kidney (C), mouse thymus (D), rat brain (E) whole cell lysates.)
Rabbit anti-Human PSAT1 Polyclonal Antibody | anti-PSAT1 antibody
Anti-PSAT1 Antibody
Western Blot (WB)
(Western blot analysis of PSAT1 expression in Hela (A), SKOV3 (B), mouse kidney (C), mouse thymus (D), rat brain (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PSAT1 expression in Hela (A), SKOV3 (B), mouse kidney (C), mouse thymus (D), rat brain (E) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of PSAT1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of PSAT1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013]
Uniprot Description
PSAT1: Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine. Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD). PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Transferase; EC 2.6.1.52; Cofactor and Vitamin Metabolism - vitamin B6
Chromosomal Location of Human Ortholog: 9q21.2
Cellular Component: cytoplasm; cytosol
Molecular Function: phosphoserine transaminase activity
Biological Process: L-serine biosynthetic process; pyridoxine biosynthetic process
Disease: Neu-laxova Syndrome 2; Phosphoserine Aminotransferase Deficiency
Research Articles on PSAT1
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Product Notes
The PSAT1 psat1 (Catalog #AAA8245859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSAT1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSAT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100. Researchers should empirically determine the suitability of the PSAT1 psat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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