Western Blot (WB)
(Western blot analysis of GLUR6 expression in SW620 (A), A549 (B), mouse brain (C) whole cell lysates.)
Rabbit GLUR6 Polyclonal Antibody | anti-GRIK2 antibody
Anti-GLUR6 Antibody
Western Blot (WB)
(Western blot analysis of GLUR6 expression in SW620 (A), A549 (B), mouse brain (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GLUR6 expression in SW620 (A), A549 (B), mouse brain (C) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GLUR6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GLUR6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation. [provided by RefSeq, Jul 2008]
Uniprot Description
GluR6: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2. Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated; Channel, calcium
Chromosomal Location of Human Ortholog: 6q16.3
Cellular Component: cell junction; dendrite cytoplasm; integral to plasma membrane; kainate selective glutamate receptor complex; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane; presynaptic membrane; terminal button
Molecular Function: extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity; ligand-gated ion channel activity; PDZ domain binding; protein homodimerization activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding
Biological Process: behavioral fear response; cellular calcium ion homeostasis; generation of action potential; glutamate signaling pathway; intracellular protein transport; ionotropic glutamate receptor signaling pathway; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, glutamatergic; neuron apoptosis; positive regulation of neuron apoptosis; positive regulation of synaptic transmission; receptor clustering; regulation of excitatory postsynaptic membrane potential; regulation of inhibitory postsynaptic membrane potential; regulation of JNK cascade; regulation of long-term neuronal synaptic plasticity; regulation of short-term neuronal synaptic plasticity; regulation of synaptic transmission; synaptic transmission; synaptic transmission, glutamatergic; transport
Disease: Mental Retardation, Autosomal Recessive 6
Research Articles on GRIK2
Similar Products
Product Notes
The GRIK2 grik2 (Catalog #AAA8245552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GLUR6 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GLUR6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the GRIK2 grik2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLUR6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
