Human IL-37 ELISA Kit | IL37 elisa kit

Human IL-37 ELISA Kit

Cat. Num. AAA8244643

• Gene Names: IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-37; Human IL-37 ELISA Kit; FIL1Z; IL1F7; IL1H4; IL1RP1; Interleukin-37; FIL1 zeta; IL-1X; Interleukin-1 family member 7; IL-1F7; Interleukin-1 homolog 4; IL-1H; IL-1H4; Interleukin-1 zeta; IL-1 zeta; Interleukin-1-related protein; IL-1RP1; Interleukin-23; IL37 elisa kit

• Reactivity: Human
• Specificity: The Human IL-37 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-37 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 218

• Applicable Applications for IL37 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for IL37 elisa kit

Principle of the Assay||The Human IL-37 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-37 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-37 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-37 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-37 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-37 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Interleukin 37 (IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

NCBI and Uniprot Product Information

• NCBI GI #: 25008593
• NCBI GeneID: 27178
• UniProt Accession #: Q9NZH6; Q56AP9; Q8TD04; Q8TD05; Q9HBF2; Q9HBF3; Q9UHA6; B5BU97
• Molecular Weight: 17,459 Da
• NCBI Official Full Name: Interleukin-37
• NCBI Official Synonym Full Names: interleukin 37
• NCBI Official Symbol: IL37
• NCBI Official Synonym Symbols: FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
• NCBI Protein Information: interleukin-37
• UniProt Protein Name: Interleukin-37
• Protein Family: Interleukin
• UniProt Gene Name: IL37
• UniProt Synonym Gene Names: FIL1Z; IL1F7; IL1H4; IL1RP1; IL-1F7; IL-1H; IL-1H4; IL-1 zeta; IL-1RP1; IL-37
• UniProt Entry Name: IL37_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL37: Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation. Belongs to the IL-1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q14.1

Cellular Component: cytoplasm; cytosol; extracellular region; extracellular space; nucleolus; nucleus

Molecular Function: cytokine activity; interleukin-1 receptor binding

Biological Process: cytokine and chemokine mediated signaling pathway; immune response; inflammatory response to antigenic stimulus