Mouse FGF21 ELISA Kit | FGF21 elisa kit

Mouse FGF21 ELISA Kit

Cat. Num. AAA8244621

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: FGF21; Mouse FGF21 ELISA Kit; Fibroblast growth factor 21; FGF-21; FGF21 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse FGF21 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse FGF21 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 210

• Applicable Applications for FGF21 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell culture supernatants, serum, plasma
• Sensitivity: < 8 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF21 elisa kit

Principle of the Assay: The MyBioSource' Mouse FGF21 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FGF21 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse FGF21 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse FGF21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

NCBI and Uniprot Product Information

• NCBI GI #: 13626695
• NCBI GeneID: 105242481
• UniProt Accession #: Q9JJN1
• Molecular Weight: 23,237 Da
• NCBI Official Full Name: Fibroblast growth factor 21
• NCBI Official Symbol: LOC105242481
• NCBI Protein Information: fibroblast growth factor 21
• UniProt Protein Name: Fibroblast growth factor 21
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf21
• UniProt Synonym Gene Names: FGF-21
• UniProt Entry Name: FGF21_MOUSE

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular region; extracellular space

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of glucose import