E2F4 blocking peptide

E2F4 Blocking Peptide

Cat. Num. AAA824318

• Gene Names: E2F4; E2F-4
• Reactivity: Human, Mouse, Rat, Chicken
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: E2F4; E2F4 Blocking Peptide; Transcription factor E2F4; E2F-4; E2F5; Transcription factor E2F5; E2F-5; E2F4 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Chicken
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 413

• Applicable Applications for E2F4 blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for E2F4 blocking peptide

The peptide is used to block Anti-E2F4 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 12669915
• NCBI GeneID: 1874
• NCBI Accession #: NP_001941.2
• NCBI GenBank Nucleotide #: NM_001950.3
• UniProt Accession #: Q16254; Q12991; Q15328; A6NGR8; B5BU56
• Molecular Weight: 43,960 Da
• NCBI Official Full Name: transcription factor E2F4
• NCBI Official Synonym Full Names: E2F transcription factor 4, p107/p130-binding
• NCBI Official Symbol: E2F4
• NCBI Official Synonym Symbols: E2F-4
• NCBI Protein Information: transcription factor E2F4; p107/p130-binding protein
• UniProt Protein Name: Transcription factor E2F4
• Protein Family: Transcription factor
• UniProt Gene Name: E2F4
• UniProt Synonym Gene Names: E2F-4
• UniProt Entry Name: E2F4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F4: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; blood circulation; regulation of cell proliferation; G1/S-specific transcription in mitotic cell cycle; organ morphogenesis; transforming growth factor beta receptor signaling pathway; epithelial cell development; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; cell volume homeostasis; regulation of cell size