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Western Blot (WB) (Western blot analysis of CNTROB expression in HepG2 (A), HUVEC (B) whole cell lysates.)

Rabbit anti-Human CNTROB Polyclonal Antibody | anti-CNTROB antibody

Anti-CNTROB Antibody

Gene Names
CNTROB; LIP8; PP1221
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
CNTROB; Polyclonal Antibody; Anti-CNTROB Antibody; LIP8; Centrobin; Centrosomal BRCA2-interacting protein; LYST-interacting protein 8; anti-CNTROB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of CNTROB protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Sequence Length
903
Applicable Applications for anti-CNTROB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CNTROB. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of CNTROB expression in HepG2 (A), HUVEC (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of CNTROB expression in HepG2 (A), HUVEC (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CNTROB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of CNTROB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-CNTROB antibody
Rabbit polyclonal antibody to CNTROB

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
97,113 Da
NCBI Official Full Name
Centrobin
NCBI Official Synonym Full Names
centrobin, centriole duplication and spindle assembly protein
NCBI Official Symbol
CNTROB
NCBI Official Synonym Symbols
LIP8; PP1221
NCBI Protein Information
centrobin
UniProt Protein Name
Centrobin
Protein Family
UniProt Gene Name
CNTROB
UniProt Synonym Gene Names
LIP8
UniProt Entry Name
CNTRB_HUMAN

NCBI Description

This gene encodes a centrosomal protein that interacts with BRCA2, and is required for centriole duplication and cytokinesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

LIP8: Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: centriole; centrosome; cytoplasm

Molecular Function: protein binding; protein domain specific binding

Biological Process: centriole replication; centrosome separation

Research Articles on CNTROB

Similar Products

Product Notes

The CNTROB cntrob (Catalog #AAA8243143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CNTROB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CNTROB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the CNTROB cntrob for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNTROB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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