Rabbit MKK6 Polyclonal Antibody | anti-MAP2K6 antibody
Anti-MKK6 Antibody
Western Blot (WB)
(Western blot analysis of MKK6 expression in Hela (A), HEK293T (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MKK6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of MKK6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
MKK6: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. Three alternatively spliced isoforms have been reported.
Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; STE group; STE7 family
Chromosomal Location of Human Ortholog: 17q24.3
Cellular Component: cytoplasm; cytoskeleton; cytosol; nucleoplasm
Molecular Function: ATP binding; MAP kinase kinase activity; protein binding; protein kinase binding; protein serine/threonine kinase activity; protein-tyrosine kinase activity
Biological Process: activation of MAPK activity; apoptosis; cardiac muscle contraction; cell cycle arrest; DNA damage induced protein phosphorylation; innate immune response; muscle cell differentiation; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; ovulation cycle process; peptidyl-tyrosine phosphorylation; positive regulation of apoptosis; positive regulation of muscle cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of prostaglandin secretion; regulation of apoptosis; regulation of mitotic cell cycle; regulation of transcription, DNA-dependent; response to drug; signal transduction; stress-activated MAPK cascade; stress-activated protein kinase signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription, DNA-dependent
