Western Blot (WB)
(Western blot analysis of HSP60 expression in Hela (A), NIH3T3 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Rabbit HSP60 Polyclonal Antibody | anti-HSPD1 antibody
Anti-HSP60 Antibody
Western Blot (WB)
(Western blot analysis of HSP60 expression in Hela (A), NIH3T3 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of HSP60 expression in Hela (A), NIH3T3 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of HSP60 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of HSP60 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Uniprot Description
HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.
Protein type: Mitochondrial; Chaperone
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: cell surface; coated pit; cyclin-dependent protein kinase activating kinase holoenzyme complex; cytoplasm; cytosol; early endosome; extracellular space; Golgi apparatus; lipid raft; lipopolysaccharide receptor complex; membrane; mitochondrial crista; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; myelin sheath; plasma membrane; protein complex; rough endoplasmic reticulum; secretory granule
Molecular Function: ATP binding; ATPase activity; chaperone binding; DNA replication origin binding; double-stranded RNA binding; insulin binding; lipopolysaccharide binding; misfolded protein binding; p53 binding; protease binding; protein binding; protein complex binding; protein heterodimerization activity; single-stranded DNA binding; ubiquitin protein ligase binding; unfolded protein binding
Biological Process: 'de novo' protein folding; B cell activation; B cell cytokine production; B cell proliferation; caspase activation; chaperone cofactor-dependent protein folding; chaperone-mediated protein complex assembly; detection of misfolded protein; isotype switching to IgG isotypes; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of neuron apoptosis; positive regulation of apoptosis; positive regulation of inflammatory response; positive regulation of interferon-alpha production; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of T cell activation; positive regulation of T cell mediated immune response to tumor cell; protein maturation; protein refolding; protein stabilization; response to ATP; response to cocaine; response to cold; response to drug; response to estrogen stimulus; response to heat; response to hydrogen peroxide; response to hypoxia; response to lipopolysaccharide; response to unfolded protein; T cell activation; viral reproduction
Disease: Leukodystrophy, Hypomyelinating, 4; Spastic Paraplegia 13, Autosomal Dominant
Research Articles on HSPD1
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Product Notes
The HSPD1 hspd1 (Catalog #AAA8242581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSP60 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP60 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/200 - 1/500). Researchers should empirically determine the suitability of the HSPD1 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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