CGNL1 sirna

CGNL1 siRNA (Mouse)

Cat. Num. AAA8240335

• Gene Names: Cgnl1; Jacop; AI503810; 4933421H10Rik; 9930020M10Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CGNL1; CGNL1 siRNA (Mouse); JACOP; KIAA1749; Cingulin-like protein 1; Junction-associated coiled-coil protein; CGNL1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CGNL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1297

• Applicable Applications for CGNL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CGNL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CGNL1 sirna

siRNA to inhibit CGNL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 749385080
• NCBI GeneID: 68178
• NCBI Accession #: NP_001291291.1
• NCBI GenBank Nucleotide #: NM_001304362.1
• UniProt Accession #: Q6AW69; Q5U5U0; Q69ZB4; Q8BLZ5; Q8BZ26; Q9D2T3
• Molecular Weight: 147,803 Da
• NCBI Official Full Name: cingulin-like protein 1
• NCBI Official Synonym Full Names: cingulin-like 1
• NCBI Official Symbol: Cgnl1
• NCBI Official Synonym Symbols: Jacop; AI503810; 4933421H10Rik; 9930020M10Rik
• NCBI Protein Information: cingulin-like protein 1
• UniProt Protein Name: Cingulin-like protein 1
• Protein Family: Cingulin-like protein
• UniProt Gene Name: Cgnl1
• UniProt Synonym Gene Names: Jacop; Kiaa1749
• UniProt Entry Name: CGNL1_MOUSE

NCBI Description

This gene encodes a protein localized to the tight junctions and adherens junctions in vertebrate epithelial cells. The encoded protein regulates the activity of Rho family GTPases during junction assembly and at confluence. At the adherens junctions, the encoded protein is part of a protein complex that links E-cadherin to the microtubule cytoskeleton. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

CGNL1: May be involved in anchoring the apical junctional complex, especially tight junctions, to actin-based cytoskeletons. Defects in CGNL1 are a cause of aromatase excess syndrome (AEXS). A disorder characterized by an estrogen excess due to an increased aromatase activity. A chromosomal aberration inv(15)(q21.2;q21.3) has been found in patients with aromatase excess syndrome. The inversion moves the promoter of the CGNL1 gene into a 5-prime position in relation to the aromatase coding region. Belongs to the cingulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Cellular Component: apical junction complex; tight junction; myosin complex; cell junction; actin cytoskeleton

Molecular Function: motor activity