RC3H1 sirna

RC3H1 siRNA (Human)

Cat. Num. AAA8240255

• Gene Names: RC3H1; RNF198; ROQUIN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RC3H1; RC3H1 siRNA (Human); KIAA2025; RNF198; Roquin-1; Roquin; RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198; RC3H1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RC3H1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1134

• Applicable Applications for RC3H1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RC3H1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RC3H1 sirna

siRNA to inhibit RC3H1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 664806080
• NCBI GeneID: 149041
• NCBI Accession #: NP_001287779.1
• NCBI GenBank Nucleotide #: NM_001300850.1
• UniProt Accession #: Q5TC82; Q5W180; Q5W181; Q8IVE6; Q8N9V1; B3KVK1
• Molecular Weight: 124,842 Da
• NCBI Official Full Name: roquin-1 isoform a
• NCBI Official Synonym Full Names: ring finger and CCCH-type domains 1
• NCBI Official Symbol: RC3H1
• NCBI Official Synonym Symbols: RNF198; ROQUIN
• NCBI Protein Information: roquin-1
• UniProt Protein Name: Roquin-1
• Protein Family: Roquin
• UniProt Gene Name: RC3H1
• UniProt Synonym Gene Names: KIAA2025; RNF198; Roquin
• UniProt Entry Name: RC3H1_HUMAN

NCBI Description

This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

RC3H1: Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; RNA-binding

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: stress granule

Molecular Function: mRNA 3'-UTR binding; zinc ion binding

Biological Process: T cell proliferation; spleen development; negative regulation of germinal center formation; negative regulation of T-helper cell differentiation; B cell homeostasis; negative regulation of activated T cell proliferation; regulation of T cell receptor signaling pathway; protein ubiquitination; T cell homeostasis; cytoplasmic mRNA processing body assembly; negative regulation of B cell proliferation; regulation of mRNA stability; lymph node development; regulation of germinal center formation