CHST4 sirna

CHST4 siRNA (Mouse)

Cat. Num. AAA8239605

• Gene Names: Chst4; GST-3; Gn6st-2; HEC-GlcNAc6ST
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHST4; CHST4 siRNA (Mouse); GST3; Carbohydrate sulfotransferase 4; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GST-3; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; HEC-GlcNAc6ST; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST-2; Gn6st-2; CHST4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHST4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 388

• Applicable Applications for CHST4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHST4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHST4 sirna

siRNA to inhibit CHST4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158749651
• NCBI GeneID: 26887
• NCBI Accession #: NP_036128.3
• NCBI GenBank Nucleotide #: NM_011998.4
• UniProt Accession #: Q9R1I1; Q9WUE5
• Molecular Weight: 44,636 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 4
• NCBI Official Synonym Full Names: carbohydrate (chondroitin 6/keratan) sulfotransferase 4
• NCBI Official Symbol: Chst4
• NCBI Official Synonym Symbols: GST-3; Gn6st-2; HEC-GlcNAc6ST
• NCBI Protein Information: carbohydrate sulfotransferase 4
• UniProt Protein Name: Carbohydrate sulfotransferase 4
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: Chst4
• UniProt Synonym Gene Names: Gst3; GST-3; HEC-GlcNAc6ST; LSST; GlcNAc6ST-2; Gn6st-2
• UniProt Entry Name: CHST4_MOUSE

Uniprot Description

CHST4: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2- branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1- 3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.-; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis; Transferase

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane; trans-Golgi network

Molecular Function: phenanthrol sulfotransferase activity; proteoglycan sulfotransferase activity; trans-3,4-dihydrodiolphenanthrene sulfotransferase activity; 2-phenanthrol sulfotransferase activity; galactose 3-O-sulfotransferase activity; 1-phenanthrol sulfotransferase activity; transferase activity; sulfotransferase activity; 3-phenanthrol sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; HNK-1 sulfotransferase activity; 4-phenanthrol sulfotransferase activity; trans-9R,10R-dihydrodiolphenanthrene sulfotransferase activity; cholesterol sulfotransferase activity; heparan sulfate 2-O-sulfotransferase activity; N-acetylgalactosamine 4-O-sulfotransferase activity; 9-phenanthrol sulfotransferase activity; heparan sulfate 6-O-sulfotransferase activity

Biological Process: sulfur metabolic process; carbohydrate metabolic process; N-acetylglucosamine metabolic process; protein amino acid sulfation; leukocyte tethering or rolling; inflammatory response