VAMP2 sirna

VAMP2 siRNA (Rat)

Cat. Num. AAA8239379

• Gene Names: Vamp2; SYB; Syb2; RATVAMPB; RATVAMPIR
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VAMP2; VAMP2 siRNA (Rat); SYB2; Vesicle-associated membrane protein 2; VAMP-2; Synaptobrevin-2; VAMP2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: VAMP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 116

• Applicable Applications for VAMP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat VAMP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VAMP2 sirna

siRNA to inhibit VAMP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981614
• NCBI GeneID: 24803
• NCBI Accession #: NP_036795.1
• NCBI GenBank Nucleotide #: NM_012663.2
• UniProt Accession #: P63045; Q64357
• Molecular Weight: 12,691 Da
• NCBI Official Full Name: vesicle-associated membrane protein 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 2
• NCBI Official Symbol: Vamp2
• NCBI Official Synonym Symbols: SYB; Syb2; RATVAMPB; RATVAMPIR
• NCBI Protein Information: vesicle-associated membrane protein 2
• UniProt Protein Name: Vesicle-associated membrane protein 2
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: Vamp2
• UniProt Synonym Gene Names: Syb2; VAMP-2
• UniProt Entry Name: VAMP2_RAT

NCBI Description

plays a role in membrane fusion in neuronal exocytosis [RGD, Feb 2006]

Uniprot Description

VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.

Protein type: Vesicle; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: zymogen granule membrane; neuron projection; synaptic vesicle membrane; clathrin-coated vesicle; intracellular membrane-bound organelle; cell; secretory granule membrane; integral to membrane; terminal button; intracellular organelle; trans-Golgi network; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; intracellular; cytoplasmic vesicle; cell junction

Molecular Function: protein C-terminus binding; calmodulin binding; identical protein binding; SNAP receptor activity; protein binding; SNARE binding; myosin binding; syntaxin binding; syntaxin-1 binding; protein complex binding; phospholipid binding; calcium-dependent protein binding

Biological Process: vesicle fusion; vesicle-mediated transport; regulation of exocytosis; synaptic vesicle exocytosis; protein transport; natural killer cell degranulation; cellular response to insulin stimulus; neurotransmitter secretion; Golgi to plasma membrane protein transport; neutrophil degranulation; response to glucose stimulus; eosinophil degranulation; protein complex assembly; calcium ion-dependent exocytosis