NDUFA12 sirna

NDUFA12 siRNA (Human)

Cat. Num. AAA8239326

• Gene Names: NDUFA12; B17.2; DAP13
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDUFA12; NDUFA12 siRNA (Human); DAP13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; 13 kDa differentiation-associated protein; Complex I-B17.2; CI-B17.2; CIB17.2; NADH-ubiquinone oxidoreductase subunit B17.2; NDUFA12 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDUFA12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 63

• Applicable Applications for NDUFA12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDUFA12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDUFA12 sirna

siRNA to inhibit NDUFA12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 385275076
• NCBI GeneID: 55967
• NCBI Accession #: NP_001245267.1
• NCBI GenBank Nucleotide #: NM_001258338.1
• UniProt Accession #: Q9UI09; Q53XX0; Q9BRV6; F8VQS7
• Molecular Weight: 7,173 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12
• NCBI Official Symbol: NDUFA12
• NCBI Official Synonym Symbols: B17.2; DAP13
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFA12
• UniProt Synonym Gene Names: DAP13; CI-B17.2; CIB17.2
• UniProt Entry Name: NDUAC_HUMAN

NCBI Description

This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

NDUFA12: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFA12 are the cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the complex I NDUFA12 subunit family.

Protein type: Mitochondrial; EC 1.6.5.3; EC 1.6.99.3; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity; electron carrier activity

Biological Process: cellular metabolic process; respiratory gaseous exchange; response to oxidative stress

Disease: Leigh Syndrome