TRIM2 sirna

TRIM2 siRNA (Human)

Cat. Num. AAA8238868

• Gene Names: TRIM2; CMT2R; RNF86
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRIM2; TRIM2 siRNA (Human); KIAA0517; RNF86; Tripartite motif-containing protein 2; E3 ubiquitin-protein ligase TRIM2; RING finger protein 86; TRIM2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRIM2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 744

• Applicable Applications for TRIM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRIM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRIM2 sirna

siRNA to inhibit TRIM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194248081
• NCBI GeneID: 23321
• NCBI Accession #: NP_001123539.1
• NCBI GenBank Nucleotide #: NM_001130067.1
• UniProt Accession #: Q9C040; O60272; Q9BSI9; Q9UFZ1; D3DP09
• Molecular Weight: 84,545 Da
• NCBI Official Full Name: tripartite motif-containing protein 2 isoform 2
• NCBI Official Synonym Full Names: tripartite motif containing 2
• NCBI Official Symbol: TRIM2
• NCBI Official Synonym Symbols: CMT2R; RNF86
• NCBI Protein Information: tripartite motif-containing protein 2
• UniProt Protein Name: Tripartite motif-containing protein 2
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM2
• UniProt Synonym Gene Names: KIAA0517; RNF86
• UniProt Entry Name: TRIM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

TRIM2: UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance. Belongs to the TRIM/RBCC family.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cytoplasm

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: regulation of neuron apoptosis; protein ubiquitination

Disease: Charcot-marie-tooth Disease, Axonal, Type 2r