IKZF4 sirna

IKZF4 siRNA (Human)

Cat. Num. AAA8238376

• Gene Names: IKZF4; EOS; ZNFN1A4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IKZF4; IKZF4 siRNA (Human); KIAA1782; ZNFN1A4; Zinc finger protein Eos; Ikaros family zinc finger protein 4; IKZF4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IKZF4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 585

• Applicable Applications for IKZF4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IKZF4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IKZF4 sirna

siRNA to inhibit IKZF4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 122891864
• NCBI GeneID: 64375
• NCBI Accession #: NP_071910.3
• NCBI GenBank Nucleotide #: NM_022465.3
• UniProt Accession #: Q9H2S9; Q96JP3
• Molecular Weight: 59,671 Da
• NCBI Official Full Name: zinc finger protein Eos
• NCBI Official Synonym Full Names: IKAROS family zinc finger 4 (Eos)
• NCBI Official Symbol: IKZF4
• NCBI Official Synonym Symbols: EOS; ZNFN1A4
• NCBI Protein Information: zinc finger protein Eos
• UniProt Protein Name: Zinc finger protein Eos
• Protein Family: Zinc finger protein
• UniProt Gene Name: IKZF4
• UniProt Synonym Gene Names: KIAA1782; ZNFN1A4
• UniProt Entry Name: IKZF4_HUMAN

NCBI Description

Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Eos, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM, Jul 2002]

Uniprot Description

IKZF4: DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Belongs to the Ikaros C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; bHLH transcription factor binding; transcription factor activity

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent