DND1 sirna

DND1 siRNA (Human)

Cat. Num. AAA8238229

• Gene Names: DND1; RBMS4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DND1; DND1 siRNA (Human); RBMS4; Dead end protein homolog 1; RNA-binding motif. single-stranded-interacting protein 4; DND1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DND1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 353

• Applicable Applications for DND1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DND1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DND1 sirna

siRNA to inhibit DND1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34740339
• NCBI GeneID: 373863
• NCBI Accession #: NP_919225.1
• NCBI GenBank Nucleotide #: NM_194249.2
• UniProt Accession #: Q8IYX4
• Molecular Weight: 38,687 Da
• NCBI Official Full Name: dead end protein homolog 1
• NCBI Official Synonym Full Names: DND microRNA-mediated repression inhibitor 1
• NCBI Official Symbol: DND1
• NCBI Official Synonym Symbols: RBMS4
• NCBI Protein Information: dead end protein homolog 1
• UniProt Protein Name: Dead end protein homolog 1
• Protein Family: Dead end protein
• UniProt Gene Name: DND1
• UniProt Synonym Gene Names: RBMS4
• UniProt Entry Name: DND1_HUMAN

NCBI Description

This gene encodes a protein that binds to microRNA-targeting sequences of mRNAs, inhibiting microRNA-mediated repression. Reduced expression of this gene has been implicated in tongue squamous cell carcinoma. Two pseudogenes of this gene are located on the long arm of chromosome 17. [provided by RefSeq, Dec 2010]

Uniprot Description

DND1: RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells. Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs. Sequence-specific RNA-binding factor that binds specifically to U-rich regions (URRs) in the 3' untranslated region (3'-UTR) of several mRNAs. Does not bind to miRNAs. May play a role during primordial germ cell (PGC) survival. However, does not seem to be essential for PGC migration.

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; nucleus

Molecular Function: miRNA binding; nucleotide binding; AU-rich element binding

Biological Process: multicellular organismal development; germ cell development