SLC4A4 sirna

SLC4A4 siRNA (Mouse)

Cat. Num. AAA8238180

• Gene Names: Slc4a4; NBC; NBC1; AI835705
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC4A4; SLC4A4 siRNA (Mouse); NBC1; NBCE1; Electrogenic sodium bicarbonate cotransporter 1; Sodium bicarbonate cotransporter; Na(+)/HCO3(-) cotransporter; Solute carrier family 4 member 4; SLC4A4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC4A4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1094

• Applicable Applications for SLC4A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC4A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC4A4 sirna

siRNA to inhibit SLC4A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 210147430
• NCBI GeneID: 54403
• NCBI Accession #: NP_001129732.1
• NCBI GenBank Nucleotide #: NM_001136260.1
• UniProt Accession #: O88343; Q3USE4; Q8BUG0; Q8QZR9; Q9R1C4
• Molecular Weight: 17,829 Da
• NCBI Official Full Name: electrogenic sodium bicarbonate cotransporter 1 isoform b
• NCBI Official Synonym Full Names: solute carrier family 4 (anion exchanger), member 4
• NCBI Official Symbol: Slc4a4
• NCBI Official Synonym Symbols: NBC; NBC1; AI835705
• NCBI Protein Information: electrogenic sodium bicarbonate cotransporter 1
• UniProt Protein Name: Electrogenic sodium bicarbonate cotransporter 1
• Protein Family: Electrogenic sodium bicarbonate cotransporter
• UniProt Gene Name: Slc4a4
• UniProt Synonym Gene Names: Nbc1; Nbce1; Sodium bicarbonate cotransporter
• UniProt Entry Name: S4A4_MOUSE

Uniprot Description

SLC4A4: Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA); also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy. Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17). Belongs to the anion exchanger (TC 2.A.31) family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; inorganic anion exchanger activity; transporter activity; symporter activity; anion transmembrane transporter activity; sodium:bicarbonate symporter activity

Biological Process: inorganic anion transport; bicarbonate transport; transport; sodium ion transport; regulation of intracellular pH; regulation of pH; ion transport; anion transport