PRPF31 sirna

PRPF31 siRNA (Human)

Cat. Num. AAA8237986

• Gene Names: PRPF31; RP11; PRP31; SNRNP61; NY-BR-99
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRPF31; PRPF31 siRNA (Human); PRP31; U4/U6 small nuclear ribonucleoprotein Prp31; Pre-mRNA-processing factor 31; Serologically defined breast cancer antigen NY-BR-99; U4/U6 snRNP 61 kDa protein; Protein 61K; hPrp31; PRPF31 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRPF31 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 499

• Applicable Applications for PRPF31 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRPF31 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRPF31 sirna

siRNA to inhibit PRPF31 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 221136939
• NCBI GeneID: 26121
• NCBI Accession #: NP_056444.3
• NCBI GenBank Nucleotide #: NM_015629.3
• UniProt Accession #: Q8WWY3; Q17RB4; Q8N7F9; Q9H271; Q9Y439; E7ESA8; F1T0A4
• Molecular Weight: 54,909 Da
• NCBI Official Full Name: U4/U6 small nuclear ribonucleoprotein Prp31
• NCBI Official Synonym Full Names: pre-mRNA processing factor 31
• NCBI Official Symbol: PRPF31
• NCBI Official Synonym Symbols: RP11; PRP31; SNRNP61; NY-BR-99
• NCBI Protein Information: U4/U6 small nuclear ribonucleoprotein Prp31
• UniProt Protein Name: U4/U6 small nuclear ribonucleoprotein Prp31
• Protein Family: U4/U6 small nuclear ribonucleoprotein
• UniProt Gene Name: PRPF31
• UniProt Synonym Gene Names: PRP31; Protein 61K; hPrp31
• UniProt Entry Name: PRP31_HUMAN

NCBI Description

This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.[provided by RefSeq, Jan 2009]

Uniprot Description

PRPF31: Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation. Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant. Belongs to the PRP31 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: Cajal body; U4/U6 x U5 tri-snRNP complex; nuclear speck; nucleus

Molecular Function: ribonucleoprotein binding; protein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome

Disease: Retinitis Pigmentosa 11