ROCK2 sirna

ROCK2 siRNA (Rat)

Cat. Num. AAA8237679

• Gene Names: Rock2; ROK; ROCK-II
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ROCK2; ROCK2 siRNA (Rat); Rho-associated protein kinase 2; Rho-associated. coiled-coil-containing protein kinase 2; Rho-associated. coiled-coil-containing protein kinase II; ROCK-II; RhoA-binding kinase 2; p150 ROK-alpha; ROKalpha; p164 ROCK-2; ROCK2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ROCK2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,388

• Applicable Applications for ROCK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ROCK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ROCK2 sirna

siRNA to inhibit ROCK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 398650620
• NCBI GeneID: 25537
• NCBI Accession #: NP_037154.2
• NCBI GenBank Nucleotide #: NM_013022.2
• UniProt Accession #: Q62868
• Molecular Weight: 160,387 Da
• NCBI Official Full Name: rho-associated protein kinase 2
• NCBI Official Synonym Full Names: Rho-associated coiled-coil containing protein kinase 2
• NCBI Official Symbol: Rock2
• NCBI Official Synonym Symbols: ROK; ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: Rock2
• UniProt Synonym Gene Names: ROCK-II; ROKalpha
• UniProt Entry Name: ROCK2_RAT

NCBI Description

a Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD, Feb 2006]

Uniprot Description

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; AGC group; DMPK family; ROCK subfamily

Cellular Component: centrosome; cytoplasm; plasma membrane; microtubule organizing center; spindle pole centrosome; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; kinase activity; ATP binding

Biological Process: chromosome condensation; negative regulation of angiogenesis; smooth muscle contraction; induction of apoptosis via death domain receptors; regulation of actin cytoskeleton organization and biogenesis; dendrite morphogenesis; neural tube closure; rhythmic process; regulation of circadian rhythm; actin cytoskeleton organization and biogenesis; centrosome duplication; regulation of keratinocyte differentiation; protein amino acid phosphorylation; Rho protein signal transduction