MRPS22 sirna

MRPS22 siRNA (Human)

Cat. Num. AAA8237554

• Gene Names: MRPS22; GIBT; GK002; C3orf5; COXPD5; RPMS22; MRP-S22
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MRPS22; MRPS22 siRNA (Human); C3orf5; RPMS22; 28S ribosomal protein S22 mitochondrial; MRP-S22; S22mt; MRPS22 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MRPS22 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 168

• Applicable Applications for MRPS22 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MRPS22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MRPS22 sirna

siRNA to inhibit MRPS22 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9910244
• NCBI GeneID: 56945
• NCBI Accession #: NP_064576.1
• NCBI GenBank Nucleotide #: NM_020191.2
• UniProt Accession #: P82650; Q9H3I1
• Molecular Weight: 18,972 Da
• NCBI Official Full Name: 28S ribosomal protein S22, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S22
• NCBI Official Symbol: MRPS22
• NCBI Official Synonym Symbols: GIBT; GK002; C3orf5; COXPD5; RPMS22; MRP-S22
• NCBI Protein Information: 28S ribosomal protein S22, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S22, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS22
• UniProt Synonym Gene Names: C3orf5; RPMS22; MRP-S22; S22mt
• UniProt Entry Name: RT22_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS22: Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). COXPD5 is an antenatal mitochondrial disease. Patients show edema, cardiomyopathy, tubulopathy, and hypotonia.

Protein type: Translation; Ribosomal; Mitochondrial

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: mitochondrion; mitochondrial small ribosomal subunit; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis

Disease: Combined Oxidative Phosphorylation Deficiency 5