CARD11 sirna

CARD11 siRNA (Mouse)

Cat. Num. AAA8236921

• Gene Names: Card11; BIMP3; CARMA1; 0610008L17Rik; 2410011D02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CARD11; CARD11 siRNA (Mouse); Caspase recruitment domain-containing protein 11; CARD11 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CARD11 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,154

• Applicable Applications for CARD11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CARD11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CARD11 sirna

siRNA to inhibit CARD11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 161086929
• NCBI GeneID: 108723
• NCBI Accession #: NP_780571.2
• NCBI GenBank Nucleotide #: NM_175362.2
• UniProt Accession #: Q8CIS0; Q4VA14; Q6KAS3; Q8BYV0
• Molecular Weight: 133,468 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 11
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 11
• NCBI Official Symbol: Card11
• NCBI Official Synonym Symbols: BIMP3; CARMA1; 0610008L17Rik; 2410011D02Rik
• NCBI Protein Information: caspase recruitment domain-containing protein 11
• UniProt Protein Name: Caspase recruitment domain-containing protein 11
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: Card11
• UniProt Entry Name: CAR11_MOUSE

Uniprot Description

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Cellular Component: T cell receptor complex; membrane; cytoplasm; plasma membrane; immunological synapse; lipid raft

Molecular Function: protein binding; CARD domain binding

Biological Process: positive regulation of cytokine production; thymic T cell selection; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; signal transduction; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; regulation of T cell differentiation; positive regulation of T cell activation