ABL2 sirna

ABL2 siRNA (Human)

Cat. Num. AAA8236678

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABL2; ABL2 siRNA (Human); ABLL; ARG; Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG; ABL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ABL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 542

• Applicable Applications for ABL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ABL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABL2 sirna

siRNA to inhibit ABL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209862772
• NCBI GeneID: 27
• NCBI Accession #: NP_001129472.1
• NCBI GenBank Nucleotide #: NM_001136000.2
• UniProt Accession #: P42684; Q5T0X6; Q5W0C5; Q6NZY6; Q7Z301; A0M8X0; B7UEF2; B7UEF3; B7UEF4; B7UEF5
• Molecular Weight: 60,864 Da
• NCBI Official Full Name: Abelson tyrosine-protein kinase 2 isoform d
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: Abelson tyrosine-protein kinase 2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; Protein kinase, TK; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extrinsic to internal side of plasma membrane; lamellipodium; dendritic spine; cytosol; phagocytic cup; actin cytoskeleton

Molecular Function: actin monomer binding; actin filament binding; protein binding; manganese ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding; protein kinase activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; regulation of cell adhesion; actin filament bundle formation; axon guidance; cell migration; peptidyl-tyrosine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; dendrite morphogenesis; protein modification process; cerebellum morphogenesis; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; signal transduction; substrate-bound cell migration, cell extension; phagocytosis; regulation of cell proliferation; regulation of apoptosis; elevation of cytosolic calcium ion concentration; Bergmann glial cell differentiation; negative regulation of Rho protein signal transduction; regulation of endocytosis; visual learning; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; neuron remodeling