SMAD5 blocking peptide

SMAD5 Blocking Peptide

Cat. Num. AAA823667

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Reactivity: Human, Mouse, Rat, Sheep
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: SMAD5; SMAD5 Blocking Peptide; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Sheep
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 465

• Applicable Applications for SMAD5 blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMAD5 blocking peptide

The peptide is used to block Anti-SMAD5 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 47778929
• NCBI GeneID: 4090
• NCBI Accession #: NP_001001419.1
• NCBI GenBank Nucleotide #: NM_001001419.2
• UniProt Accession #: Q99717; O14688; Q15798; Q9UQA1
• Molecular Weight: 52,258 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; MAD, mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
• UniProt Entry Name: SMAD5_HUMAN

NCBI Description

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ubiquitin protein ligase binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; embryonic pattern specification; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; transforming growth factor beta receptor signaling pathway; cartilage development; erythrocyte differentiation; osteoblast fate commitment; cardiac muscle contraction