CRYM sirna

CRYM siRNA (Rat)

Cat. Num. AAA8236520

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRYM; CRYM siRNA (Rat); Ketimine reductase mu-crystallin; CDK108; NADP-regulated thyroid-hormone-binding protein; CRYM sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CRYM siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 313

• Applicable Applications for CRYM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CRYM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRYM sirna

siRNA to inhibit CRYM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758840
• NCBI GeneID: 117024
• NCBI Accession #: NP_446407.1
• NCBI GenBank Nucleotide #: NM_053955.1
• UniProt Accession #: Q9QYU4
• Molecular Weight: 33,554 Da
• NCBI Official Full Name: ketimine reductase mu-crystallin
• NCBI Official Synonym Full Names: crystallin, mu
• NCBI Official Symbol: Crym
• NCBI Protein Information: ketimine reductase mu-crystallin
• UniProt Protein Name: Ketimine reductase mu-crystallin
• Protein Family: Ketimine reductase
• UniProt Gene Name: Crym
• UniProt Entry Name: CRYM_RAT

NCBI Description

human homolog may play a role in hearing [RGD, Feb 2006]

Uniprot Description

CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.

Protein type: Oxidoreductase; Enzyme, misc.; Transcription, coactivator/corepressor; Mitochondrial; Lyase; EC 1.5.1.25

Cellular Component: mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; hormone binding; NADP binding; transcription corepressor activity

Biological Process: response to vitamin D; sensory perception of sound; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process; mitochondrial transport