GUCA1B sirna

GUCA1B siRNA (Mouse)

Cat. Num. AAA8236317

• Gene Names: Guca1b; GCAP2; BC018258
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GUCA1B; GUCA1B siRNA (Mouse); Guanylyl cyclase-activating protein 2; GCAP 2; Guanylate cyclase activator 1B; GUCA1B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GUCA1B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 201

• Applicable Applications for GUCA1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GUCA1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GUCA1B sirna

siRNA to inhibit GUCA1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22122571
• NCBI GeneID: 107477
• NCBI Accession #: NP_666191.1
• NCBI GenBank Nucleotide #: NM_146079.1
• UniProt Accession #: Q8VBV8
• Molecular Weight: 23,520 Da
• NCBI Official Full Name: guanylyl cyclase-activating protein 2
• NCBI Official Synonym Full Names: guanylate cyclase activator 1B
• NCBI Official Symbol: Guca1b
• NCBI Official Synonym Symbols: GCAP2; BC018258
• NCBI Protein Information: guanylyl cyclase-activating protein 2
• UniProt Protein Name: Guanylyl cyclase-activating protein 2
• Protein Family: Guanylyl cyclase-activating protein
• UniProt Gene Name: Guca1b
• UniProt Synonym Gene Names: GCAP 2
• UniProt Entry Name: GUC1B_MOUSE

Uniprot Description

GCAP2: Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure. Defects in GUCA1B are the cause of retinitis pigmentosa type 48 (RP48). RP48 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

Protein type: Calcium-binding

Cellular Component: photoreceptor inner segment; photoreceptor outer segment; membrane; plasma membrane

Molecular Function: calcium sensitive guanylate cyclase activator activity; metal ion binding; calcium ion binding; guanylate cyclase regulator activity

Biological Process: visual perception; regulation of guanylate cyclase activity; response to stimulus; phototransduction; positive regulation of guanylate cyclase activity