BRD4 sirna

BRD4 siRNA (Mouse)

Cat. Num. AAA8236113

• Gene Names: Brd4; Brd5; MCAP; HUNK1; WI-11513
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BRD4; BRD4 siRNA (Mouse); MCAP; Bromodomain-containing protein 4; Mitotic chromosome-associated protein; BRD4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BRD4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 723

• Applicable Applications for BRD4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BRD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BRD4 sirna

siRNA to inhibit BRD4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226342873
• NCBI GeneID: 57261
• NCBI Accession #: NP_065254.3
• NCBI GenBank Nucleotide #: NM_020508.4
• UniProt Accession #: Q9ESU6; Q8VHF7; Q8VHF8; B0V2V7
• Molecular Weight: 80,605 Da
• NCBI Official Full Name: bromodomain-containing protein 4 isoform 1
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: Brd4
• NCBI Official Synonym Symbols: Brd5; MCAP; HUNK1; WI-11513
• NCBI Protein Information: bromodomain-containing protein 4
• UniProt Protein Name: Bromodomain-containing protein 4
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: Brd4
• UniProt Synonym Gene Names: Mcap; MCAP
• UniProt Entry Name: BRD4_MOUSE

NCBI Description

This gene was temporarily named bromodomain-containing 5 (Brd5) and was renamed bromodomain-containing 4 (Brd4). [provided by RefSeq, Jul 2008]

Uniprot Description

BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; ATYPICAL group; BRD family

Cellular Component: transcription elongation factor complex b; condensed nuclear chromosome; nuclear chromatin; nucleolus; chromosome; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of RNA elongation from RNA polymerase II promoter; chromatin modification; protein amino acid phosphorylation; inner cell mass cell proliferation; positive regulation of DNA binding; chromosome segregation