LSM2 sirna

LSM2 siRNA (Human)

Cat. Num. AAA8236079

• Gene Names: LSM2; G7B; snRNP; C6orf28; YBL026W
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LSM2; LSM2 siRNA (Human); C6orf28; G7B; U6 snRNA-associated Sm-like protein LSm2; Protein G7b; Small nuclear ribonuclear protein D homolog; snRNP core Sm-like protein Sm-x5; LSM2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LSM2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 95

• Applicable Applications for LSM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LSM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LSM2 sirna

siRNA to inhibit LSM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 10863977
• NCBI GeneID: 57819
• NCBI Accession #: NP_067000.1
• NCBI GenBank Nucleotide #: NM_021177.4
• UniProt Accession #: Q9Y333; Q6FGG1
• Molecular Weight: 10,835 Da
• NCBI Official Full Name: U6 snRNA-associated Sm-like protein LSm2
• NCBI Official Synonym Full Names: LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
• NCBI Official Symbol: LSM2
• NCBI Official Synonym Symbols: G7B; snRNP; C6orf28; YBL026W
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm2
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm2
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM2
• UniProt Synonym Gene Names: C6orf28; G7B
• UniProt Entry Name: LSM2_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM2: Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing. Identified in the spliceosome C complex. LSm subunits form a heteromer with a doughnut shape. Belongs to the snRNP Sm proteins family.

Protein type: RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; Ral GTPase binding; protein kinase binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA catabolic process, deadenylation-dependent decay